Dataset on Survival Response and mRNA Expression of Drosophila melanogaster Exposed to Aqueous Datura stramonium Leaf Extract

Food was replaced every four days to prevent contamination and nutritional degradation.For the gene expression analysis, adult flies were preserved in TRIzol reagent at the end of the exposure period. Total RNA was extracted using the TRIzol method, quantified spectrophotometrically, and used for cDNA synthesis. Semi-quantitative PCR was conducted to assess the expression levels of Eiger, Wingless, Dpp, and Glob1.

GAPDH was employed as the housekeeping gene for normalization. Gene-specific primers were used, amplification was performed for 30 cycles, and PCR products were resolved on 1% agarose gel. Band intensities were quantified using ImageJ software to generate relative expression values.This dataset provides raw and processed data suitable for research in toxicology, stress response genetics, environmental health, molecular biology, and the study of plant-derived bioactive compounds using D. melanogaster as a model organism.