Whole genome sequecing of strain PAMC 27389 and four type strains of genus Pseudorhodobacter

The terrestrial soil sample was preserved at -80C until the use. For cultivation, a serially diluted aliquot (100 µl) of the sample in 0.85% NaCl (w/v) was spread on R2A (BD Difco) plates and incubated at 15C for 11 days.

Pure cultures of the bacterial isolates obtained were deposited to Polar and Alpine Microbial Collection (PAMC, Lee et al., 2012) and preserved as glycerol suspensions (20% in distilled water, v/v) at -80 C. In this study, one of these strains, PAMC 27389T, was routinely cultured on 0.5 % TYS at 15C after the determination of optimal temperature for growth. For comparing with strain PAMC 27389T, four type strains of Pseudorhodobacter species, P. wandonensis KCTC 23672T, P. antarcticus KCTC 23700T, P. aquimaris KCTC 23043T, and P. ferrugineus LMG 22047T were purchased from Korean Collection of Type Cultures (KCTC) and Laboratory of Microbiology Gent Bacteria Collection (LMG) and used as reference strains following cultivation under comparable conditions as PAMC 27389T.