Data · collection · 2018
Accessible quantitative phase imaging in confocal microscopy with sinusoidal-phase synthetic optical holography
Listed in DataCite
We present a technically simple implementation of quantitative phase imaging in confocal microscopy based on synthetic optical holography with sinusoidal-phase reference waves.
Description
Using a Mirau interference objective and low-amplitude vertical sample vibration with a piezo-controlled stage, we record synthetic holograms on commercial confocal microscope (Nikon, model: A1R; Zeiss: model: LSM-880), from which quantitative phase images are reconstructed.
We demonstrate our technique by stain-free imaging of cervical (HeLa), ovarian (ES-2) cancer cells and stem cell (mHAT9a) samples. Our technique has the potential to extend fluorescence imaging applications in confocal microscopy by providing label-free cell finding, monitoring cell morphology as well as non-perturbing long-time observation of live cells based on quantitative phase contrast.
Links
Where it is published
- Repository landing page figshare.com/collections/Accessible_quantitative_phase_imaging_in_confocal_… ↗
landing page · from DataCite
- DOI doi.org/10.6084/m9.figshare.c.4283600.v1 ↗
DOI / persistent id · from DataCite
Documentation and papers
- CC BY 4.0 creativecommons.org/licenses/by/4.0 ↗
license · from DataCite
- IsSupplementTo 10.1364/ao.58.000a55 doi.org/10.1364/ao.58.000a55 ↗
publication · from DataCite
Catalogue records · 2
- DataCite API api.datacite.org/dois/10.6084/m9.figshare.c.4283600.v1 ↗
metadata API · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.6084/m9.figshare.c.4283600.v1 ↗
catalogue entry · from DataCite
Topics
- Stated by source
- Clinical medicine · Electrical engineering, electronic engineering, information engineering
- Inferred from text
- Cancer 75% · Image 65% · Imaging 75% · Microscopy 75% · Ophthalmology and optometry 75%
Related
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.6084/m9.figshare.c.4283600.v1 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| concepts[disease].local:disease:cancer | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3212 | enrichment · DataCite | taxonomy-embedding@1.1.0 | title+keywords+description (75%) |
| concepts[field].fos:clinical-medicine | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:electrical-engineering-electronic-engineering-information-engineering | source · DataCite | connector:datacite@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:imaging | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:microscopy | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · DataCite | connector:datacite@1.0.0 | |
| description | source · DataCite | connector:datacite@1.0.0 | /data/attributes/descriptions |
| license | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| publication_date | source · DataCite | connector:datacite@1.0.0 | /data/attributes/dates |
| title | source · DataCite | connector:datacite@1.0.0 | /data/attributes/titles/0/title |
| updated_date | source · DataCite | connector:datacite@1.0.0 |