Assembling and Mining the Genomes of Giant Antarctic Foraminifera

Furthermore, the work will further develop a molecular toolkit which could open up new avenues of research on the physiology, ecology, and population dynamics of this important group of Antarctic organisms. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. Educational experiences related to the "thrill of scientific exploration and discovery" for students and the general public will be provided through freely-available short films and a traveling art/science exhibition.

The project will also provide hands-on research opportunities for undergraduate students. Explorers Cove, situated on the western shore of McMurdo Sound, harbors a unique population of foraminiferan taxa at depths accessible by scuba diving that otherwise are primarily found in the deep sea. The project will use next-generation DNA sequencing and microdissection methods to obtain and analyze nuclear and mitochondrial genomes from crown members of two species each from three distinct, early-evolving foraminiferal groups.

It will also use next generation sequencing methods to characterize the in-situ prokaryotic assemblages (microbiomes) of one of these groups and compare them to reference sediment microbiomes. The phyogenomic studies of the targeted Antarctic genera will help fill significant gaps in our current understanding of early foram evolution. Furthermore, comparative genomic analyses of these six species are expected to yield a better understanding of the physiology of single-chambered agglutinated forams, especially the bioadhesive proteins and regulatory factors involved in shell composition and morphogenesis.

Additionally, the molecular basis of cold adaptation in forams will be examined, particularly with respect to key proteins.