RNA sequencing analysis of LPS-treated BMDMs from Nrp2fl/flLyz2-cre and Nrp2fl/fl mice

After 7 days, BMDMs were treated with 200 ng/ml LPS for 18 h in vitro. After polarization, BMDMs were collected for RNA sequencing analysis.

All the animal experiments were approved by Institutional Animal Care and Use Committee of Sichuan University.UID RNA-seq experiment and high through-put sequencing and data analysis were conducted by Seqhealth Technology Co., LTD (Wuhan, China). Briefly, total RNAs were extracted from samples using TRIzol Reagent. 2 μg total RNAs were used for stranded RNA sequencing library preparation using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® following the manufacturer’s instruction. Raw sequencing data was first filtered by Trimmomatic (version 0.36).