Experimental study on the inhibition of excessive inflammation of macrophages by postnatal elements of Lactobacillus reuteri

Method (1) Orthogonal combination of Zhibai Formula (ZBZF) and Lactobacillus reuteri (LR) co culture was used to prepare extracellular vesicles. (2) CCK-8 detects the toxicity of different concentrations of extracellular vesicles on macrophages.

(3) Low (Drug-L), medium (Drug-M), and high (Drug-H) concentrations were used to intervene in macrophage inflammation models. Biochemical assay kits were used to detect NO content, qRT PCR was used to detect TNF - α, IL-6, IL-1 β, and miRlet-7c-5p expression, and ELISA was used to detect TNF - α and IL-6 secretion in cell culture media. Result (1): The prebiotic activity and synergistic LR antibacterial activity of ZBZF No. 6 were significant.