Data · dataset · 2023
Figure 6_raw data
Listed in Borealis
Description
Raw Image data that were analysed to be presented in Figure 6. If image data is not of interest, see doi.org/10.5683/SP2/0SHJNT where we uploaded the final data plotted in Figure 6. Here see "Figure6_SummarizeColocImageData.xlsx" for summary of Image data including cell line used, BH3 mimetics tested and size of datasets.
This helps navigate the subfolders to find the images of interest. Data was organised by date of the experiment. Within each folder see, '.Tiffs' exported from PerkinElmer Harmony software.
Read the rest (3 more)
Files names for example "r02c02f01p01-ch1..." indicating the image is from row 2, column 2, field of view 1, position 1 and channel 1. Within each folder see "Analysis" Sub folder where one can find: -"SaveImagingConfiguration.xlsx" where we saved the imaging settings from the Phenix microscope. The objective lens, excitation/emission settings for channels 1,2,3,4 (in order that they appear), and number of fields of view acquired for that experiment can be found here. -.xlsx file with "platemap" included in filename.
Shows detailed map of Cells/Treatment within 384 well plate. Use Row/Column information to navigate the image data. -Subfolder labelled "CP_results" : include results the object-level (single cell) results exported from Cell Profiler. These data were plotted separately in GraphPad Prism (see .pzf file) to determine the states o from each replicate.
Then, median values were plotted in Figure 6 from 3+ replicates. See "Figure6_CombinedFinalColocResults.xlsx" where we combine the final results ultimately plotted in Figure 6.
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.5683%2FSP3%2FB0DGGH ↗
landing page · from borealisdata ca
- DOI doi.org/10.5683/sp3/b0dggh ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.5683%2FSP3%2… ↗
metadata API · from borealisdata ca
Topics
- Stated by source
- Other
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.5683/SP3/B0DGGH | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[field].dataverse_subject:other | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[modality].local:modality:image | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:imaging | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |