Data for: Impacts of large herbivores on mycorrhizal fungal communities across the Arctic

Fungal DNA was extracted from soil samples collected at herbivore exclosure experiments in 15 sites around the Arctic to evaluate the impact of large mammalian herbivores on mycorrhizal fungal communities. The data includes DNA sequence reads amplified using ITS1m-LR5 and SSU515fngs-AML2 long-read primer pairs targeting general fungal sequences and arbuscular mycorrhizae, respectively.

Sequences were taxonomically assigned through the UNITE database using PlutoF, and the MaarjAM database and assigned to fungal guild using FUNguild. The pipeline from trimmed, demultiplexed sequence reads to taxonomic and functional assignment is included in the Rscript "EcographySubmission_SynthesisScript.R". Soil properties, date of exclosure establishment, and general metadata is included in the file "MetaData.csv", and percent plant functional type cover is included for 11 sites in the file "Cover_data.csv".