<p>Principal component analysis (PCA) of 278,855 unlinked SNVs.</p>

<p>The samples differentiated in genotypic space, as visualized with PCA. Each species forms a distinct cluster separated along PC1 and PC2. Known hybrid individuals fall intermediate to the parental species clusters, including the laboratory-generated F1 <i>S. haematobium × S. bovis</i> hybrid [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014625#ppat.1014625.ref067" target="_blank">67</a>] and natural <i>S. curassoni × S. bovis</i> hybrids [<a href="plospathogens.org/article/info:doi/10.1371/journal.ppat.1014625#ppat.1014625.ref019" target="_blank">19</a>].

Samples are colored according to their ITS genotypes and marker shapes are used to identify the mitotype. All individuals collected from cattle presented homozygous <i>S. bovis</i> ITS alleles. Miracidia from humans presented <i>S. haematobium</i>, <i>S. curassoni</i>, or heterozygous <i>S. haematobium/S. curassoni</i> ITS alleles.