Antitumor Compounds from Cordyceps Militaris

Seven monomeric compounds, N6-(2-hydroxyethyl) adenosine (HEA), ergosterol (E), ergosta-7,22-diene-3,5,6-triol (EI), 5α,8α-epidioxy-(22 E ,24 R )-ergosta-6,22-dien-3β-ol (ED), ergosta-7,22-dien-3β,5α-dihydroxy-6-one(EO),(20 S ,22 E ,24 R )-Eegosta-7,22-dien-3β,5α,6β,9α-tetraol22 E ,24 R -ergosta-7,22-diene-3β,5α,6β,9α-tetraol (ET), and (24 S )-5,22-stigmastadien-3β-ol (SE), were harvested using different solvents, and the structure of each compound was identified.

The activities and functions of the isolated compounds were tested by label-free, real-time cell analysis methods at the cellular level, and their antitumor effects were verified using mouse models of Lewis and H22 tumors. The anti-insomnia effect of HEAadenosine was tested in an anti-insomnia mouse model. The interactions between E and 8 A549 cell proteins were determined.