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Data · dataset · 2024

Replication data for: Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses

Listed in International Potato Center

Pathogen-tested in vitro plants of the Peruvian sweetpotato landrace ‘Huachano’ (accession no. CIP420065) were obtained from the germplasm collection of the International Potato Center (CIP, Lima, Peru) and transferred to an insect-proof greenhouse at CIP.

Description

The isolate of Sweetpotato feathery mottle virus SPFMV (SPFMV-Piu) and Sweetpotato chlorotic stunt virus SPCSV (SPCSV m2–47) used for plant inoculation in this study were originally obtained from sweetpotato plants infected in the field in Peru (Cuellar et al., 2008).

They were maintained in I. setosa plants in an insect-proof greenhouse at CIP, Lima, Peru. The viruses were inoculated to sweetpotato plants via grafting. Infection was confirmed by index grafting onto the susceptible indicator plants of I. setosa and double or triple antibody sandwich enzyme linked immunosorbent assay for SPFMV and SPCSV respectively as described in Kreuze et al. (2008).

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Where it is published

Catalogue records · 1

Topics

Stated by source
Agricultural Sciences
From keywords
Life Sciences
Provenance · 1 source records, 10 field assertions
SourceKeyLast seenRaw
International Potato Centerdoi:10.21223/P3/NKWPGE12 d agoJSON v1
FieldAssertionExtractorEvidence
concepts[field].anzsrc:group:3102enrichment · data cipotato orgtaxonomy-embedding@1.1.0title+keywords+description (72%)
concepts[field].dataverse_subject:agricultural-sciencessource · data cipotato orgconnector:data_cipotato_org@1.0.0/subjects
concepts[field].local:field:life-sciencesmapping · data cipotato orgconnector:data_cipotato_org@1.0.0/subjects
concepts[modality].local:modality:sequencingenrichment · data cipotato orgkeyword-concept-rules@1.0.0title+description (75%)
created_datesource · data cipotato orgconnector:data_cipotato_org@1.0.0
descriptionsource · data cipotato orgconnector:data_cipotato_org@1.0.0/description
publication_datesource · data cipotato orgconnector:data_cipotato_org@1.0.0
titlesource · data cipotato orgconnector:data_cipotato_org@1.0.0/name
updated_datesource · data cipotato orgconnector:data_cipotato_org@1.0.0
version_labelsource · data cipotato orgconnector:data_cipotato_org@1.0.0