Effect of eIF5A K49 acetylation status on the translatome of Toxoplasma gondii

Using the ME49 strain as the genetic background, we performed ribosome profiling (Ribo-seq) on three distinct variants: wild-type parasites, an acetylation-mimic mutant (eIF5A-K49Q), and a deacetylation-mimic mutant (eIF5A-K49R).

Parasites were cultured under either standard conditions (non-stressed tachyzoites) or alkaline stress conditions (pH 8.2 medium for 4 days) to induce in vitro bradyzoite differentiation. Three biological replicates were included for each group, resulting in a total of 18 samples. By sequencing ribosome-protected mRNA fragments, this dataset enables genome-wide characterization of ribosome occupancy and translational output, providing a valuable resource for understanding how eIF5A-K49 acetylation reshapes translational programs during developmental stage conversion in Toxoplasma gondii.