Supplementary data – Global transcriptomic response of Malassezia pachydermatis isolates under ketoconazole-induced stress – Domán, 2026

Despite reduced susceptibility to azole antifungals has increasingly been reported in M. pachydermatis, the molecular mechanisms underlying azole stress responses in this species remain poorly understood. In this study, we investigated the global transcriptional response of two M. pachydermatis isolates with different ketoconazole minimum inhibitory concentration values following short-term ketoconazole exposure.

Ketoconazole treatment resulted in slightly distinct transcriptional profiles in the two isolates. In isolate 12372, 80 genes were significantly upregulated and 120 were downregulated, whereas isolate 12693 showed 158 upregulated and 211 downregulated genes. Both isolates exhibited significant upregulation of genes involved in ergosterol biosynthesis, including ERG3, ERG5, ERG11, ERG24, and ERG25 suggesting a conserved compensatory response to azole-mediated disruption of sterol metabolism.