Comprehensive analysis of miRNA expression, secretion profile, and functional network during myogenesis in response to spaceflight

Two types of high-throughput sequencing were performed in parallel: 1) Small RNA sequencing was performed simultaneously with cell precipitation and culture supernatant, using Illumina TruSeq Small RNA kit for library construction and Solexa platform sequencing. The ACGT101-miR v4.2 process was used to standardize data, and Fisher's exact test was used to screen for differentially expressed miRNAs. The screening criteria were log2FC>1 and p<0.05; After sequencing and filtering out non coding interfering RNA, miRNA annotation was completed by aligning miRBase 22.1. 2) Synchronize SMART seq full-length mRNA sequencing of cell samples, synthesize full-length cDNA with trace RNA using SMARTer kit, fragment and construct library, and perform dual end sequencing on Illumina Novaseq 6000 platform; The offline data was subjected to quality control, genome alignment, and transcript assembly.

FPKM was used to quantify expression, and edgeR was used to screen for differential mRNA (| FC |>2, p<0.05). The dataset includes the appendix: Appendix 1 shows the differential expression of miRNAs (DEmiRNAs) in C2C12 cells between the space culture group and the ground control group; Table 2 shows the differential mRNA (DEGs) expression data of candidate target genes for C2C12 intracellular differentially expressed miRNAs (DEGmiRNAs) screened by SMART seq sequencing; Table 3 shows the differential expression of miRNAs (DEmiRNAs) in C2C12 culture medium between the space culture group and the ground control group.