Identification and functional study of the antimicrobial peptide, QUB- 1985, from the skin secretion of the large odorous frog, Odorrana livida

Also, the haemolysis assay found that no more than 18% of red blood cells were affected at at the concentration of the highest MIC value (64 μM). The peptide had an HC10 of 30.89 μM, which indicated a remarkably low cytotoxicity. QUB-1985 also exerted anti-proliferation activity on non-small cell lung cancer cells (NCI-H838) at concentrations of 10-5 M and above, and the IC50 was 1.470×10-5 M. From the above, QUB-1985 is competent to become an antimicrobial lead compound, especially towards Gram-negative bacteria and its anti-proliferation activity may broaden its utility into basic cancer research.

Also, following the same templates in molecular cloning, may obtain novel AMP classes from Odorrana frogs with potent antimicrobial effects that could widen the resource of antibiotic agents.<br> <br><i>Thesis embargoed until 31 December 2026</i>.