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Data · collection · 2015

Phenolic, flavonoid contents, anticholinesterase and antioxidant evaluation of Iris germanica var ; florentina

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This study was designed to investigate antioxidant and anticholinesterase potential of Iris germanica var ; florentina .

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Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory potential of plant samples were investigated by Ellman’s assay. Antioxidant activity was performed using DPPH, H 2 O 2 and ABTS free radical scavenging assays.

Total phenolics and flavonoids contents were expressed in mg GAE/g dry weight and mg RTE/g, respectively. In AChE inhibition assay, Ig.Fl, Ig.Sp and Ig.Cf fractions exhibited highest activity with IC 50 values of < 0.1, 5.64 and 19 μg/mL, respectively. In BChE inhibitory assay, Ig.Fl, Ig.Sp, Ig.Cf and Ig.Cr were most active with IC 50 of < 0.1, < 0.1, 31 and 78 μg/mL, respectively.

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In DPPH assay, Ig.Fl and Ig.Cf exhibited highest inhibition of free radicals, 80.52% (IC 50 = 9 μg/mL) and 78.30% (IC 50 = 8 μg/mL), respectively. In ABTS assay Ig.Cr, Ig.Cf, Ig.Fl and Ig.Sp exhibited IC 50 values of < 0.1, 2, 2 and 3 μg/mL, respectively.

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