Data · dataset · 2024

Data associated with the manuscript Superiority of chromosomal compared to plasmid-encoded compensatory mutations.

Listed in University of Liverpool Research Data Catalogue

Ecological dynamics of plasmid compensatory mutations: raw data.

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The abstract submitted with the preprint is pasted below: Plasmids are important vectors of horizontal gene transfer in microbial communities but can impose a burden on the bacteria that carry them. Such plasmid fitness costs are thought to arise principally from conflicts between chromosomal- and plasmid-encoded molecular machineries, and thus can be ameliorated by compensatory mutations (CMs) that reduce or resolve the underlying causes.

CMs can arise on plasmids (i.e. plaCM) or on chromosomes (i.e. chrCM), with contrasting predicted effects upon plasmid success and subsequent gene transfer because plaCM can also reduce fitness costs in plasmid recipients, whereas chrCM can potentially ameliorate multiple distinct plasmids. Here, we develop theory and a novel experimental system to directly compare the ecological effects of plaCM and chrCM that arose during evolution experiments between Pseudomonas fluorescens SBW25 and its sympatric mercury resistance megaplasmid pQBR57.

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We show that while plaCM was predicted to succeed under a broader range of parameters in mathematical models, chrCM dominated in our experiments, including conditions with numerous recipients, due to a more efficacious mechanism of compensation, and advantages arising from transmission of costly plasmids to competitors (plasmid ‘weaponisation’). We show analytically the presence of a mixed Rock-Paper-Scissors regime for CMs, driven by trade-offs with horizontal transmission, that offers one possible explanation for the observed failure of plaCM to dominate even in competition against an uncompensated plasmid.

Our results reveal broader implications of plasmid-bacterial evolution for plasmid ecology, demonstrating the importance of specific compensatory mutations for resistance gene spread. One consequence of the superiority of chrCM over plaCM is the likely emergence in microbial communities of compensated bacteria that can act as ‘hubs’ for plasmid accumulation and dissemination. Analysis scripts associated with these data are available at github.com/jpjh/COMPMUT_dynamics

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From keywords: Earth & Environmental Science

Provenance · 1 source records, 5 field assertions

Source	Key	Last seen	Raw
University of Liverpool Research Data Catalogue	oai:datacat.liverpool.ac.uk:2585	14 h ago	JSON v1

Field	Assertion	Extractor	Evidence
concepts[field].local:field:earth-environmental	mapping · datacat liverpool ac uk	connector:datacat_liverpool_ac_uk@1.0.0
description	source · datacat liverpool ac uk	connector:datacat_liverpool_ac_uk@1.0.0	/metadata/dc/description
license_text	source · datacat liverpool ac uk	connector:datacat_liverpool_ac_uk@1.0.0
publication_date	source · datacat liverpool ac uk	connector:datacat_liverpool_ac_uk@1.0.0
title	source · datacat liverpool ac uk	connector:datacat_liverpool_ac_uk@1.0.0	/metadata/dc/title

Data associated with the manuscript Superiority of chromosomal compared to plasmid-encoded compensatory mutations.

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