Manual nuclei segmentations in near-isotropic, aligned volume electron microscopy (eFIB-SEM) of Mosquito stylet (jrc_mosquito-stylet-6)

<p dir="ltr"><b>Annotation description</b>: Dense segmentations of nuclei in jrc_mosquito-stylet-6 using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.</p><p dir="ltr"><b>Annotation protocol</b>: Using Amira to manually segment organelles in vEM for machine learning V.3 (<a href="dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3" target="_blank">dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3</a>)</p><p dir="ltr"><b>Software</b>: Amira 3D 2021.1, Classic Segmentation Workroom</p><p dir="ltr"><b>Label voxel size (nm)</b>: 32 x 32 x 32 (x, y, z)</p><p dir="ltr"><b>Reconstructed data dimensions (µm)</b>: 50.88 x 44.22 x 295.01 (x, y, z)</p><p dir="ltr"><b>Classes annotated</b>: Nucleus</p><p dir="ltr"><b>Source dataset (EM) ID</b>: jrc_mosquito-stylet-6</p><p dir="ltr"><b>Source dataset (EM) voxel size (nm)</b>: 8 x 8 x 8 (x, y, z)</p><p dir="ltr"><b>Source dataset (EM) data dimensions (µm)</b>: 50.88 x 44.23 x 295.02 (x, y, z)</p><p dir="ltr"><b>Source dataset (EM) DOI</b>: <a href="doi.org/10.25378/janelia.33050051" target="_blank">doi.org/10.25378/janelia.33050051</a></p><p dir="ltr"><b>Dataset URL</b>: <a href="" target="_blank">s3://janelia-cosem-datasets/jrc_mosquito-stylet-6/jrc_mosquito-stylet-6.zarr/labels/inference/segmentations/</a></p><p dir="ltr"><b>Visualization website</b>: <a href="openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6" target="_blank">openorganelle.janelia.org/datasets/jrc_mosquito-stylet-6</a></p>