Transcriptome of Mycobacterium tuberculosis under oxidative stress conditions

After treatment with 10 mM H ₂ O ₂ for 4 hours, Mtb cells were collected at OD ₆₀₀=0.4 and immediately frozen and ground in liquid nitrogen. Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's instructions.

Subsequently, Ribo clean rRNA Deletion Kit Mega (Vazyme) was used to remove ribosomal RNA. The chain specific RNA library was constructed using the VAHTS Universal V8 RNA seq Library Prep Kit for Illumina (Vazyme) and sequenced on the Illumina NovaSeq 6000 platform, obtaining a 150 bp double ended read length.