<p>Comparison of RhGB07 with other sarbecoviruses.</p>

The insets highlight regions of interest of the RBD that are either involved in binding of ACE2 or have presented structural changes across clades. <b>(B)</b> The C-α trace of the RBD of RhGB07 rendered as balls-and-sticks superimposed with the RBD of PRD-0038 apo or PRD-0038 bound to R. Alcyone ACE2 (PDB: 8U0T).

The insets show a zoomed-in view of two regions of the RBD that show the greatest changes in the position of the C-α of the RBD. The region containing the ins1-loop and Δ4-loop coincides with putative ACE2-interacting residues based on mapping the equivalent residues. <b>(C)</b> RBD (top) and NTD (bottom) of RhGB07 cartoon depicted with the cryo-EM density contoured in this region. Residues comprising a putative fatty acid binding pocket as seen in SARS-CoV-2 RBD [<a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.ref039" target="_blank">39</a>] or a putative biliverdin binding pocket as seen in other coronavirus NTD [<a href="plosbiology.org/article/info:doi/10.1371/journal.pbio.3003944#pbio.3003944.ref040" target="_blank">40</a>] are highlighted.