Quantitative Transcriptomic and Metabolic Analyses Reveal the Roles of RpoS and Crp in the Acid Resistance System 1 in Escherichia coli

Therefore, it is necessary to investigate the acid resistance mechanism of intestinal bacteria. Although RpoS and Crp have long been found to regulate the acid resistance of E. coli, there is a lack of systematic research to analyze its regulatory network under the acidic environment.

Through an integrated approach combining genomic analysis, we obtained robust research findings. These results establish a novel theoretical framework for understanding microbial adaptation to acidic environments, while offering potential applications for developing new antibiotic targets against intestinal pathogens and engineering industrial bacterial strains with enhanced acid tolerance capabilities.