Illumina 16s amplicon sequencing data for in-situ Macquarie Island Mesocosm assessing the toxicity of residual hydrocarbons.

Automated Ribosomal Intergenic Spacer analysis (ARISA) was conducted for both Fungal and Bacterial soil communities across all extractions (n = 270). ARISA results confirmed uniformity of replicate extractions. One replicate for each sample was selected for further work (n=90).

Illumina sequencing was conducted with the 90 samples and these samples correspond with those also selected for microfluidic qPCR. See Thesis in publications section for more details. This data was processed in Mothur, using only the forward read.

Missing samples are due to quality control, and duplicate samples are due to re-run in a subsequent Illumina submission. Three spreadsheets are included; - OTU abundance by sample, which is the key output document following processing in Mothur - OTU taxonomy classification, which identifies each OUT - Illumina with Enviro for Primer, which is a processed copy of the first document, together with key environmental data, for use in multivariate analysis and cross-referencing with microfluidic qPCR data . 'Environmental' tab provides additional data on samples, including TPH data and vegetation observations at one time point (expressed as percentage of coverage).

Contact Sarah Houlahan for more information on chemical data. OTU = operational taxonomic unit. Sample Set/Action ### Date ### Weeks after spiking Establishment ### 17/01/2013 ### -45 weeks P1 ### 01/02/2013 ### -43 weeks P2 ### 30/10/2013 ### -3 weeks Spiking ### 19-25/11/2013 ### 0 weeks T1 ### 10/12/2013 ### 2 weeks T2 ### 30/03/2014 ### 18 weeks T3 ### 07/01/2015 ### 59 weeks T4 ### 24/03/2015 ### 69 weeks