Omics · study · 2026
Nobiletin targets USP5 to inhibit ferroptosis and alleviate doxorubicin-induced cardiotoxicity
Listed in NCBI GEO
Doxorubicin (DOX) is an effective chemotherapeutic agent that can cause cardiotoxicity and myocardial injury.
Description
Nobiletin (NOB), a natural polymethoxyflavone, has been reported to exert protective effects in cardiovascular disease models. To investigate the transcriptional changes associated with DOX-induced cellular injury and the potential protective effects of NOB, RNA sequencing was performed in H9c2 rat cardiomyoblasts subjected to different treatments.
H9c2 cells were divided into four groups: control (CON), nobiletin-treated (NOB), doxorubicin-treated (DOX), and doxorubicin plus nobiletin-treated (DOX+NOB) groups, with three biological replicates per group. Cells were treated with 100 μM NOB and/or 1 μM DOX for 24 h, according to the experimental group. Transcriptomic profiles were generated using paired-end sequencing on an Illumina NovaSeq 6000 platform.
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The resulting RNA-seq dataset provides a resource for characterizing transcriptional responses to DOX exposure and investigating the potential molecular effects of NOB in the context of DOX-induced cellular injury.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344097 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344097 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1514614 ncbi.nlm.nih.gov/bioproject/PRJNA1514614 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- Cardiovascular disease 75% · Disease 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE344097 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cardiovascular-disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |