The 4C In planta Quantitative Biotinylproteomics Studies Reveal a WInd-Related Kinase 1 (WIRK1) Functioning as An Early Signaling Component in Wind-induced Thigmomorphogenesis and Gravitropism

Largely unknown is how plants transduce these versatile wind force signals downstream to nuclear events and to the development of thigmomorphogenic phenotype or anemotropic response. To identify molecular components at the early steps of the wind drag force signaling, two mechanical signals-regulated phosphoproteins, identified from our previous phosphoproteomic study of Arabidopsis touch response, mitogen-activated protein kinase kinase 1 (MKK1) and 2 (MKK2), were selected for performing in planta TurboID (ID)-based proximity-labeling (PL) proteomics.

This quantitative biotinylproteomics was separately performed on MKK1-ID and MKK2-ID transgenic plants, respectively, using the TurboID overexpression transgenics as a universal control. This quantitative biotinylproteomic work successfully identified 11 and 71 MKK1- and MKK2- associated proteins, respectively. The raw data and mascot search results were summarized and uploaded here.