<p>Comparison between the infecting assay and cross assay.</p>

<p>DropMap-measured affinity (K_D) of IgG-SC from subclinical patients infected with <b>(A)</b> DENV1 or <b>(B)</b> DENV2. <b>(A-B)</b> Data are divided by categories of IgG-SC that bind one sE-dimer or both sE-dimers, and the nature of the DropMap assay, i.e., “infecting” or “cross”, as indicated in the legend. Horizontal bars represent median and interquartile range. Each dot represents one IgG-SC recognizing at least one sE-dimer.

The dotted line indicates the 30nM affinity limit that discriminates E protein binders from non-binders. <i>p</i> values are calculated using Kruskall-Wallis followed by with Dunn’s multiple comparison test. ns, not significant; *, p ≤ 0.05; **, p ≤ 0.01; ****, p ≤ 0.0001. N.B.: non-binding. <i>Note</i>: The “infecting” assay contains sE and sE-dimer antigens of the serotype infecting the patient analyzed, and the sE-dimer of the other serotype (i.e., non-infecting).