Scaffold characterization data, cellular assay data, and in vivo evaluation data in animals.

This dataset contains the raw experimental data and statistical analysis data for the paper “Effects of Microcapsules with Different Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem Cells and In Vivo Evaluation.” In the experiments, CNF/SA microcapsules with different matrix stiffness were prepared by adjusting the mass ratio of carbon nanofibers (CNFs) to sodium alginate (SA), and their physicochemical properties were characterized using particle size statistics, scanning electron microscopy, Fourier transform infrared spectroscopy, texture analysis, and in vitro degradation experiments.

Subsequently, the effects of microcapsules with different stiffness on the survival and osteogenic differentiation of mesenchymal stem cells were evaluated by Live/dead staining, ALP activity assays, immunofluorescence staining, and other methods. A subcutaneous implantation model in SD rats was used, and the in vivo histological response was evaluated by H&E, Masson, and Alizarin Red S staining as well as immunohistochemical analysis of OPN and OCN.