N-3 polyunsaturated fatty acids prevent diabetic cardiomyopathy by promoting TET2-mediated DNA demethylation

N-3 polyunsaturated fatty acids (n-3 PUFAs) improved insulin resistance and cardiovascular function, however the underlay molecular mechanism is unknown. The mice were fed with high-fat diet and injected with STZ (55mg/kg/d) for 7 consecutive days.

Diabetes mice were fed with high-fat diet for another 12 weeks to induce diabetes DCM, DCM mice were then divided into four groups: DCM group (normal saline); metformin group (MET, 150 mg/kg/d metformin buffer); low-dose n-3 PUFAs group (Ln-3, 150 mg/kg/d); high-dose n-3 PUFAs group (Hn-3, 300 mg/kg/d). The control group (CON) was fed with a standard diet (normal saline). Fasting serum concentrations of insulin, TC, TG, LDL-C, and the atherogenic index of plasma were significantly lower in both Ln-3 and Hn-3 groups than in DCM group. Total n-3 PUFAs and n-3/n-6 n-3 PUFAs ratio in heart were significantly lower in DCM group than them in CON, Ln-3 and Hn-3 groups.