Data · collection · 2021
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)
Listed in NASA Earthdata CMR
Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia).
Description
Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below.
Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples:
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- SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL
- SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL
- SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL
- SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL
- SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL
- SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL
Links
Get the data
- Download the dataset. data.aad.gov.au/eds/5068/download ↗
download · from NASA CMR
Where it is published
- DOI doi.org/10.26179/5e33a1e1e8c87 ↗
DOI / persistent id · from NASA CMR
Documentation and papers
- data.aad.gov.au /aadc/metadata/citation.cfm?entry_id=AAS_4406_BP_MP_2005 ↗
Citation reference for this metadata record and dataset.
documentation · from NASA CMR
- Public information for AAS project 4406 secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4406 ↗
project · from NASA CMR
Catalogue records · 2
- CMR UMM-JSON cmr.earthdata.nasa.gov/search/concepts/C1693329360-AU_AADC.umm_json ↗
metadata API · from NASA CMR
- CMR record cmr.earthdata.nasa.gov/search/concepts/C1693329360-AU_AADC.html ↗
catalogue entry · from NASA CMR
Topics
- Stated by source
- Automated DNA Sequencer · FIELD SURVEYS · LABORATORY · Microfauna · Microflora · Not provided
- Inferred from text
- Sequencing 75% · Soil sciences 70%
Provenance · 1 source records, 16 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NASA Earthdata CMR | C1693329360-AU_AADC | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[field].anzsrc:group:4106 | enrichment · NASA CMR | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[instrument].gcmd_instrument:ads | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/land-surface/soils/microfauna | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[measured_variable].gcmd:earth-science/land-surface/soils/microflora | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · NASA CMR | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[platform].gcmd_platform:field-surveys | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[platform].gcmd_platform:laboratory | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| concepts[platform].gcmd_platform:not-provided | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| description | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/Abstract |
| license_text | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| publication_date | source · NASA CMR | connector:nasa_cmr@1.0.0 | |
| spatial | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/SpatialExtent |
| temporal | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/TemporalExtents |
| title | source · NASA CMR | connector:nasa_cmr@1.0.0 | /umm/EntryTitle |
| version_label | source · NASA CMR | connector:nasa_cmr@1.0.0 |