Video 1_Sleep fragmentation frequency is associated with distinct immune, microbial, and barrier-related profiles in DSS-induced colitis in mice.mp4

This study examined endpoint-specific responses to low- and high-frequency sleep fragmentation in DSS-induced colitis in mice.</p>Methods<p>Male C57BL/6 mice were assigned to Control, DSS, DSS with low-frequency sleep fragmentation (DSS+LSF), DSS with high-frequency sleep fragmentation (DSS+HSF), or high-frequency sleep fragmentation alone (HSF).

Colitis was induced with 2% DSS for 7 days. Sleep fragmentation was applied for 12 h/day during the light phase at 60 sweeps/h for LSF or 240 sweeps/h for HSF. Clinical indices, histology, epithelial barrier-associated proteins, inflammatory and stress-related markers, colonic CD4⁺ T-cell subsets, fecal microbiota composition, and fecal SCFAs were assessed.</p>Results<p>Clinical and macroscopic indices varied across experimental groups, but corrected comparisons among DSS-treated groups were not significant.