Raw data for article "Phenotypes of synovial fluid Treg cells in checkpoint blockade-related inflammatory arthritis"

Unsupervised clustering revealed two atypical immune-activating Treg cell clusters common to both CBIA and RA patients, in which a immuno-activating (featuring ICOS+CD134+CD137+) cluster was distinct to CBIA patients. This immuno-activating cluster was found to have a positive correlation to the mCDAI in CBIA patients. In single-cell secreting proteomics of SF-derived Treg cells in CBIA patients, we found that clusters distinct to the immuno-activating cell group featured inflammatory cytokine secretion of mainly MCP1 and MCP4, which was validated by Peripheral CBIA secreting proteomics (vs. RA, which preferentially secreted CCL11/CXCL10).

Other non-immuno-activating cells mainly secreted immune-modulatory cytokines of IL10, IL4, and TGFB1. Consistently, MCP1/MCP4+polysecreting cluster proportion was also positively correlated with mCDAI. At single-cell proteomic level, an atypical, MCP1/MCP4+polysecreting immuno-activating Treg cell type is found to have a strong relation to clinical disease activity of CBIA.This dataset contains three datasets of single-cell membrane /secreting proteomics data (located within three folders respectively), and each folder contains the original values of protein expression (.csv files), analysis codes (.Rdata, .txt files), and grouping information (.xlsx files).