Data · dataset · 2026
Exploring the Mechanism of Gansui-Gancao Pair in Treating Malignant Ascites Based on Network Pharmacology and Animal Experiments - Raw Data
Listed in ScienceDB
This study aims to explore the mechanism of Kansui Radix-Glycyrrhizae Radix et Rhizoma (KR-GRR) herb pair on malignant ascites (MA) by using network pharmacology and animal experiment.
Description
Firstly, the active components of KR-GRR herb pair and the corresponding targets were screened from the TCMSP database. The common targets and signaling pathways of KR-GRR herb pair in treating MA were predicted by network pharmacology, and a protein-protein interaction network was constructed to form a visualized map of Chinese Tradition medicine-components-diseases-targets.
GO and KEGG analyses were used to predict the underlying mechanism of KR-GRR herb pair in treating MA. Secondly, molecular docking was employed to obtain the binding ability of the active components of KR-GRR herb pair to the core targets. Finally, the MA mice model was developed using H22 liver cancer cells for the mechanism validation.
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As a result, network pharmacology results indicated that 13 core bioactive components and 51 potential targets of KR-GRR against MA, with key targets including serine/threonine-protein kinase 1 (AKT1), tumor necrosis factor (TNF), interleukin- 6 (IL-6), tumor protein 53 (TP53), IL-1β, and Matrix metallopeptidase 9 (MMP9). A total of 141 signaling pathways were screened out by KEGG analysis, and molecular docking results suggested that the PI3K/AKT1/MMP9 signaling pathway played a pivotal role in the therapeutic effects of KR-GRR against MA.
The animal experiment demonstrated that KR-GRR herb pair significantly improved the general condition, survival rate, and peritoneal histopathological damage in MA mice. Additionally, 2.5 g/kg of KR-GRR reduced abdominal circumference and ascites volume, and suppressed the expression of AKT1 and MMP9 in ascitic fluid. Furthermore, KR-GRR downregulated the mRNA levels of AKT1, TNF, IL-6, TP53, IL-1β, and MMP9 in peritoneal tissues, as well as the protein expression of p-PI3K, PI3K, p-AKT1, AKT1, and MMP9, especially the ratios of p-PI3K/PI3K and p-AKT1/AKT1.
Taken together, KR-GRR herb pair may exert therapeutic effects against MA by inhibiting the activation of the PI3K/AKT1/MMP9 signaling pathway.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.nprd.000cv ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Medicine & Health · Social Science
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.nprd.000cv | 9 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |