Integrated analysis of transcriptome and proteome reveal that PDCoV infection induces autophagy-dependent ferroptosis to facilitate viral replication

However, the interactions between PDCoV and host cells remain poorly understood. In this study, we employed transcriptomic and proteomic analyses to investigate host responses to PDCoV infection.

Our results identified 1,448 differentially expressed genes (DEGs) at 1.5 h post-PDCoV infection and 11,753 DEGs, along with 898 differentially expressed proteins (DEPs) at 18 h post-PDCoV infection. Furthermore, several signaling pathways, including innate immunity, autophagy, and ferroptosis, were primarily enriched following an integrated analysis of the transcriptome and proteome. Protein-protein interaction (PPI) analysis indicated that proteins closely associated with these pathways, such as IFIT1, MX2, ISG15, RSAD2, OASL, ATG16, and GPX4, were central to the interaction network.