Dataset of the Simultaneous Determination of Seven Nicotinic Acid and Its Derivatives in Different Rice Varieties by Liquid Chromatography–Tandem Mass Spectrometry

The mobile phase conditions that can make the peak shapes of the two target compounds sharp and symmetrical were selected. Figure 3 shows the effect of different extraction conditions on the extraction efficiency of 7 types of nicotinic acid and its derivatives. Different gradients of methanol volume fraction, extraction temperature, extraction time, and extraction solvent volume were set to compare the extraction efficiency of nicotinic acid and its derivatives under different conditions.

The methanol volume fraction, extraction temperature, extraction time, and extraction solvent volume with the best extraction efficiency were selected Figure 4 shows the matrix effects and recovery rates of 7 types of nicotinic acid and its derivatives under different purification conditions. Firstly, HLB, HC-C18, and PSA+C18+GCB purification materials were selected, and their matrix effects were calculated to select the optimal purification material; Based on the optimal purification material, different methanol volume fractions were set for solid-phase extraction column loading conditions, and the matrix effects of the target substance corresponding to each methanol volume fraction were calculated to select the optimal loading conditions; On the basis of the optimal loading system, different solid phase extraction conditions with different methanol volume fractions were set, and the matrix effect of the target compound corresponding to each methanol volume fraction was calculated to select the optimal elution conditions; Finally, standard solutions were added to the optimal solid-phase extraction loading and elution conditions to calculate the compound recovery rate and verify the reliability of the optimal purification conditions. Figure 5 shows the matrix effect of seven nicotinic acids and derivatives in six types of rough Rice noodles matrices after purification by HLB solid phase extraction.

In order to verify the practicability of the optimized HLB solid phase extraction column purification conditions, the wide applicability of the purification method is verified by purifying the six types of rough Rice noodles matrix extracts and calculating their matrix effect. The data file in Table 3 shows the recoveries and relative standard deviations of seven nicotinic acids and derivatives in six types of rice matrices at three spiked levels.

The accuracy of the method is verified by adding low, medium and high standard solutions to six types of brown Rice noodles and calculating the spiked recoveries. Table 5 The data file shows the actual content of nicotinic acid and derivatives in 343 portions of brown Rice noodles The data file in Figure 6 compares the content of niacin and its derivatives in six types of rice and other foods. By searching existing literature data, the differences in the content of niacin and its derivatives in common foods were compared, highlighting the advantage of niacin and its derivatives content in brown rice.