Oxford Nanopore direct RNA sequencing data from MHV-A59 wild-type and nsp15 H262A mutant samples

<p dir="ltr">This dataset contains Oxford Nanopore direct RNA sequencing data generated from mouse hepatitis virus A59 (MHV-A59) samples, including wild-type and nsp15 H262A mutant virus samples.</p><p dir="ltr">Five RNA samples were multiplexed and sequenced on the same Oxford Nanopore RNA004 flow cell: a mock sample, MHV-wt 1, MHV-wt 3, MHV-mut 1, and MHV-mut 3. Total RNA was extracted using TRIzol reagent, followed by poly(A) RNA enrichment using a VAHTS poly(A) enrichment workflow.

Libraries were prepared for Oxford Nanopore direct RNA sequencing using the RNA004 workflow with a custom reverse-transcription-adapter (RTA) barcoding strategy.</p><p dir="ltr">Sequencing was performed on flow cell FAZ51928 from 6–9 March 2026. Reads were demultiplexed using Warpdemux. The deposited dataset contains raw POD5 signal data together with associated sequencing outputs, sample metadata, and documentation describing sample identities and barcode assignments.</p><p dir="ltr">The dataset was generated to support analyses of MHV RNA, including viral transcript profiles and poly(A) tail characteristics.