Data · dataset · 2025
Rodents , cytb, mitochondrion
Listed in ScienceDB
Total DNA was extracted from the feces using the QIAamp DNA Stool Mini Kit (QIAGEN, Germany), following the manufacturer’s protocol.
Description
Based on the complete mitochondria DNA (mtDNA) of N. confucianus (NC_023960.1) and A. agrarius (HM034866.1), we designed the primer (forward primer: ACTAATGACATGAAAAATCATCGTT; reverse-primer: TTCTTCATTTTTGGTTTACAAGACC) for the CYTB region using Primer Premier (V 5.0). We used the ABI3730XL gene analyzer (PE Applied Biosystems, USA) for bidirectional gene sequencing.
Assembled sequences were subjected to BLAST analysis against the NCBI (National Center for Biotechnology Information) database, and species identification was determined using a percent identity threshold (PI) and query coverage (QC).
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.29591 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.29591 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[modality].local:modality:sequencing | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |