Lipid Transfers in the Canadian Arctic

The fraction was then subdivided using the Motoda splitting box until reaching ca 1000 mg which was deposited on a pre-burned GF/C filter and stored in pre-burned aluminium foil at -80°C until lipid analysis. The carbon content of copepod species was estimated based on the length-mass relationships established by Forest et al. (2011). At each station, 12L of water was collected at the surface and at the subsurface chlorophyll maximum in Niskin-type bottles mounted on a rosette sampler.

A set of probes was used to measure the physico-chemical parameters of seawater. The pH of seawater was measured with a spectrophotometer, nutrient data were analyzed aboard from fresh samples, an aliquot was preserved in Lugol acid for taxonomic analysis and seawater filtration(3L) was conducted for further analyses of lipid contents. Phase separation methods with chloform:methanol:water was used for lipid extractions and lipid classes and fatty acid was determined using thin-layer chromatography and gas chromatography with flame ionization detection.