Data for: "Scale-down bioreactors reveal that prolonged mixing times deteriorate the performance of antibody-producing Chinese hamster ovary cells"

The work explores two approaches of prolonged mixing time in laboratory-scale vessels without changing the power input. Experiments in 3 liter bioreactors were performed with NISTCHO mammalian cells producing monoclonal antibodies called NISTmAb.

The data set includes at-line data such as glucose, lactate, viable cell density and product concentration as well as antibody glycosylation pattern analysis with HPLC, whole mRNA transcriptomic (performed extern) and metabolome analysis with HPLC coupled ESI-Triple quadrupole mass spectrometry.