Liver transcriptome data of T2DM rats treated with MOTS-C and aerobic exercise

RNA seq technology was used to obtain liver transcriptome data for each group of rats. Through differential expression gene analysis, GO/KEGG enrichment analysis, and protein interaction network construction, key metabolic pathways and core genes were screened. Preliminary data shows that both MOTS-C and aerobic exercise intervention alone can partially reverse abnormal expression of gluconeogenesis, lipid synthesis, and inflammation related genes in the liver of T2DM rats; The combined intervention showed a synergistic effect, significantly enriched in the AMPK, PPAR, and insulin signaling pathways.

Consistent with previous literature, exercise training can improve mitochondrial function by upregulating PGC-1 α, and this study found that MOTS-C can further enhance this effect. In addition, the combined intervention specifically regulated multiple novel long non coding RNAs, suggesting the existence of an epigenetic regulatory mechanism of motor MOTS-C cross-talk. The transcriptome data of this project provides important resources for revealing the molecular basis of MOTS-C simulation and enhancing exercise benefits, and also provides new ideas for developing "exercise mimetics" combination therapies for T2DM.