Omics · study · 2026
Wave-forming Nanotexture-Mediated Priming of Stem Cell for Osteogenesis via Mechanotransduction Machinery and Chromatin reprogramming [ATAC-seq]
Listed in NCBI GEO
Biophysical cues such as the topography of ECM can direct cell lineage specification and accelerate the regeneration of cultured tissue.
Description
Here, we show how changes in the cellular and nuclear morphologies of mesenchymal stem cells induced by clinically applicable nanotextures influence the conformation of the cells’ chromatin and their osteogenic differentiation. The wave-forming nanotextures (W-NT) were eco-friendly fabricated on medical-grade titanium by a non-thermal femto-second laser.
W-NT impacted cellular elongation, nuclear architecture, and chromatin reprogramming via microtubules. The ensuing chromatin reprogramming enhanced the cells’ responsiveness to osteogenic differentiation biochemical factors, revealed by ATAC and RNA sequencing. Mechanistically, osteogenesis was enhanced by the Yap-mediated mechanotransduction machinery, involving ‘nanotexture’ - ‘microtubules’ - ‘nucleus deformation’ - ‘Yap-RUNX2 axis and histone 3 acetylation activity’.
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In rabbits with bone defects, implants with nanotextures enhanced bone regeneration without exogenous signaling molecules. Our findings suggest that nanotopographical modification of medical devices could be leveraged to facilitate bone regeneration through nuclear-shaped mediated chromatin priming.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE253nnn/GSE253081 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE253081 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1063832 ncbi.nlm.nih.gov/bioproject/PRJNA1063832 ↗
project · from NCBI GEO
Topics
- Stated by source
- Genome binding/occupancy profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE253081 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:genome-binding-occupancy-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |