Imaging · dataset · 2018
Functions of FACT in Breaking Nucleosome and Maintaining Its Integrity at Single-nucleosome Level
Listed in Teesside University Research Data Repository
Fig 3i Gel-shift assay shows the properties of FACT, SSRP1 or SPT16 to promote the deposition of H2A/H2B dimer into tetrasome to form nucleosome.
Description
Fig 4a Gel-shift assay shows the DNA binding properties of SSRP1 and SSRP1ΔHMG. Fig 4b Gel-shift assay shows the properties of SSRP1 and SSRP1ΔHMG to promote H2A/H2B dimer deposition onto tetrasome to form nucleosome.
Fig S1a EM images of the reconstituted nucleosomes used for the study. Fig S2a SDS-PAGE analysis of the purified FACT and its individual subunits Fig S3a EM images of the reconstituted mono-tetrasomes
Links
Where it is published
- DOI doi.org/10.17632/9tc87th633.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Medicine & Health · Microscopy · Molecular imaging · Social Science
- Inferred from text
- Image 65%
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/9tc87th633.1 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].anzsrc:field:340605 | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Electron Microscopy'] |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:microscopy | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Electron Microscopy'] |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |