PARSIFAL gut metagenomic sequencing data (Swedish population)

To better understand the lifestyle and environmental drivers of the microbiome and resistome we analyzed 74 stool samples from the cross-sectional PARSIFAL study (doi.org/10.1111/j.1398-9995.2005.00939.x) using paired-end shotgun metagenomic sequencing. The study population consisted of children aged 5-13 years living in the Uppsala and Järna regions (Sweden) and entailed participants living on farms, children with anthroposophic lifestyles and two reference groups of children living in the same areas but not following these lifestyles.

For this study, all 74 fecal samples of the Swedish part of the PARSIFAL study were obtained. Samples were taken by the parents, first stored at –20 °C, and finally stored at –80 °C before DNA extraction, as described by Dicksved et al. (doi.org/10.1128/AEM.02223-06). The participant data and samples that were used for the analyses in this study were collected between October 2000 and May 2002.