Data · dataset · 2019
Laser micro-irradiation experiment for Y14-silenced U2OS cells
Listed in Teesside University Research Data Repository
U2OS cells that stably express the GFP-tagged MDC1, Ku70 or Ku80 were transiently transfected with or without the Y14 shRNA-mCherry-expressing vectors.
Description
Cells were subjected to laser-microirradiation with 405 nm UV, followed by live-cell imaging using a confocal microscopy. The representative confocal images of each experimental condition were in the folder "sequential_images".
Images of the green fluorescence show accumulation of GFP-fusion proteins at sites of laser-microirradiation at indicated time points. Images of the red fluorescence show the expression of mCherry as the indication of shY14-expressing cells. Fluorescence intensities of GFP-fusion proteins at irradiated region in each tested cells were quantified periodically, normalized, and presented as mean ± S.D. for at least 10 cells in each experiment.
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Quantitative results were summarized in ".xlsx" files.
Links
Where it is published
- DOI doi.org/10.17632/yym8r56fgf.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science
- Inferred from text
- Image 65% · Imaging 75% · Microscopy 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/yym8r56fgf.1 | 10 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:imaging | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:microscopy | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |