Spatially and temporally resolved metabolome of the oral human cavity, negative ionization mode, batch 1

When subject id, time of collection, and oral location for blank and QC sample type are equal to 0. RESULTS: Many the metabolites that exhibit heterogeneous distribution, several important metabolites are linked to oral health, such as N-acetylated metabolites, L-ornithine, saccharides, and guanosine monophosphate. Next to the spatial distribution of metabolites, we identified known and novel time dependencies.

Among the novel diurnal metabolites, N-acetyl methionine, N,N,N-trimethyl lysine, and N-acetyl tryptophan revealed diurnal patterns in all oral locations. Where the former two behave similarly to cortisol, and the latter has its nadir in the M. Moreover, a pool of amino acids and mono-phosphorylated nucleotides manifested a significant diurnal rhythm exclusively in BT.