Data · dataset · 2025
Raw datasets from canine adipose-derived mesenchymal stem cells proteomics cultured under conditions of hypoxia (DFO), inflammation (IFN-gamma) or both.
Listed in Teesside University Research Data Repository
Study question: The DFO, IFN-gamma and both can change proteome of canine adipose tissue-derived MSCs (cAT-MSCs)?
Description
What data shows: These data show the cAT-MSCs profile proteomic of 4 experimental groups, control (n = 6), DFO (n = 6), IFN-gamma and IFN-gamma+DFO (n = 6). In the control group, cells were maintained in DMEM/F-12 containing 1% Pen-Strep and 1% Fungizone (base medium).
The DFO group consisted of cells maintained in base medium + 50 µmol of DFO. In the IFN-gamma group, cells were maintained in base medium containing 50 ng/ml IFN-gamma. In the IFN-gamma+DFO group, cells were maintained in base medium containing 50 µmol of DFO and 50 ng/ml IFN-gamma.
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In all groups, cultures were conducted in an incubator at 37.5 °C, with 5% CO2 in air and 100% humidity for 48 hours. After harvesting, cAT-MSCs collected and were separated of the culture medium by centrifugation and prepared individually for proteomic analysis by ESI Q-Tof mass spectrometer. How the data can be interpreted: There are four folders in the dataset, separated by control, DFO, IFN-gamma and IFN-gamma+DFO, with all quantified cell proteins, per culture, properly identified.
Links
Where it is published
- DOI doi.org/10.17632/gdg29rw3sr.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Life Sciences · Mass spectrometry
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/gdg29rw3sr.1 | 15 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:mass-spectrometry | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Proteomics'] |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |