Data · dataset · 2024
Exploring the mRNA and Plasma Protein Levels of BDNF, NT4, SIRT1, HSP27, and HSP70 in Multiple Sclerosis Patients and Healthy Controls
Listed in Teesside University Research Data Repository
Multiple Sclerosis sclerosis (MS) is a chronic, autoimmune neurodegenerative disease affecting the central nervous system.
Description
It is a major cause of non-traumatic neurological disability among young adults in North America and Europe. This study focuses on neuroprotective genes (BDNF, NT4/5, SIRT1, HSP70, and HSP27).
Gene expression and protein levels of these markers were compared between MS patients and healthy controls. BThe blood samples were collected from 42 patients with multiple sclerosis (MS) and 48 control subjects without MS. Quantitative real-time PCR was performed to measure the expression of specific genes.
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The samples were analyzed in duplicate, and the abundance of mRNA was quantified using the 2-ΔCt method. ELISA assay was used to measure the concentration of specific proteins in the plasma samples. Results The results show that a 3.5-fold decreased in the gene expression of BDNF by 3.5-fold corresponds to a 1.5-fold downregulation in the associated plasma protein concentration (P < 0.001).
Similar trends were observed with NT-4 (5five-fold decrease, slight elevation in protein), SIRT1 (two2-fold decrease, two2-fold protein decrease), HSP70 (4four-fold increase, nearly 2two-fold protein increase), and HSP27 (four4-fold increase, two2-fold protein increase) (P < 0.001). The This study reveals strong correlations between gene expression and protein concentration in MS patients, emphasizing the relevance of these neuroprotective markers in the disease.
Links
Where it is published
- DOI doi.org/10.17632/f4s2sgdz58.2 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Gene expression · Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/f4s2sgdz58.2 | 12 h ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:field:310505 | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Gene Expression'] |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |