Table 1_A conserved structural framework accompanies evolutionary divergence and expression specialization in the grapevine SKP1 family.xlsx

Although SKP1-mediated regulation is essential for diverse plant processes, the evolutionary diversification and regulatory specialization of SKP1 family members in grapevine remain poorly understood.</p>Methods<p>In this study, genome-wide identification, structural characterization, phylogenetic analysis, promoter analysis, transcriptome profiling, and subcellular localization assays were integrated to investigate the diversification of the grapevine SKP1 family.</p>Results<p>Eleven VvSKP1 genes were identified in Vitis vinifera and characterized at structural, evolutionary, regulatory, and expression levels.

Although individual VvSKP1 members displayed differences in protein architecture and evolutionary relationships, most retained conserved SKP1-like motifs and structural features, indicating preservation of a common molecular framework. Phylogenetic analysis across eight representative angiosperms revealed three major SKP1 lineages and suggested lineage-specific diversification among retained family members. Promoter analyses revealed distinct transcription factor-binding landscapes among VvSKP1 genes, and grapevine TF DAP-seq datasets identified potential MYB-associated regulation of specific members.