Data · dataset · 2026
Data from: Plant-produced viral nanoparticles decorated with nanobodies against HER2 improve retention and recruitment of immune cells in solid tumors
Listed in DataCite
Potato virus X (PVX), a filamentous, positive-sense RNA plant virus, has been engineered into a molecular tool for diverse biotechnological applications, including cancer cell targeting.
Description
Here, we present the production and functional characterization of genetically-encoded PVX-derived nanoparticles decorated with nanobodies targeting two common receptors in human cancer cells, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
We first generated a series of PVX-derived nanoparticles displaying distinct nanobodies against EGFR and HER2 in Nicotiana benthamiana plants. Self-assembly and structural integrity of the recombinant nanoparticles were confirmed by immunogold electron microscopy. We next characterized in vitro the cancer-cell binding capacity of the different recombinant viral nanoparticles (VNPs) by flow cytometry and confocal microscopy.
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Best performing VNPs were further assayed in vivo using tumor-bearing mice. Results showed that nanobody decoration can increase retention time and myeloid cell recruitment in the tumor microenvironment in HER2+ mouse tumor models in vivo. In conclusion, nanobody-displaying PVX-derived nanoparticles may constitute a new promising plant-produced biotechnological product for cancer immunotherapy.
Links
Where it is published
- Repository landing page datadryad.org/dataset/doi:10.5061/dryad.x69p8czzn ↗
landing page · from DataCite
- DOI doi.org/10.5061/dryad.x69p8czzn ↗
DOI / persistent id · from DataCite
Documentation and papers
- Creative Commons Zero v1.0 Universal creativecommons.org/publicdomain/zero/1.0/legalcode ↗
license · from DataCite
- IsCitedBy 10.1002/adhm.202505936 doi.org/10.1002/adhm.202505936 ↗
publication · from DataCite
Catalogue records · 2
- DataCite API api.datacite.org/dois/10.5061/dryad.x69p8czzn ↗
metadata API · from DataCite
- DataCite Commons commons.datacite.org/doi.org/10.5061/dryad.x69p8czzn ↗
catalogue entry · from DataCite
Topics
- Stated by source
- Biological sciences · Chemical sciences · Health sciences · Medical biotechnology
- From keywords
- Biomedical engineering
- Inferred from text
- Cancer 75% · Microscopy 75%
Provenance · 1 source records, 14 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DataCite | 10.5061/dryad.x69p8czzn | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| byte_size | source · DataCite | connector:datacite@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].fos:biological-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:chemical-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:health-sciences | source · DataCite | connector:datacite@1.0.0 | |
| concepts[field].fos:medical-biotechnology | source · DataCite | connector:datacite@1.0.0 | |
| concepts[modality].local:modality:microscopy | enrichment · DataCite | keyword-concept-rules@1.0.0 | title+description (75%) |
| created_date | source · DataCite | connector:datacite@1.0.0 | |
| description | source · DataCite | connector:datacite@1.0.0 | /data/attributes/descriptions |
| license | source · DataCite | connector:datacite@1.0.0 | /data/attributes/rightsList |
| publication_date | source · DataCite | connector:datacite@1.0.0 | /data/attributes/dates |
| title | source · DataCite | connector:datacite@1.0.0 | /data/attributes/titles/0/title |
| version_label | source · DataCite | connector:datacite@1.0.0 |