Omics · study · 2026
Cell Membrane Maintenance by PhoP is Critical for Methyylglyoxal Resistance in Mycobacterium tuberculosis
Listed in NCBI GEO
The human pathogen Mycobacterium tuberculosis primarily infects macrophages.
Description
Upon activation by IFNγ, infected macrophages produce higher levels of methylglyoxal, a toxic aldehyde. Mycobacterium tuberculosis defense mechanisms against aldehydes have not been elucidated.
A transposon mutagenesis screen identified 22 loci required for resistance to methylglyoxal, one of which was phoP. The attenuated phoP mutant was even more attenuated in mice that accumulate MG. Secondary mutations in the fatty acid β-oxidation genes fadE25 or fixB restored methylglyoxal resistance to a phoP mutant by restoring the membrane barrier that is compromised in a phoP mutant.
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Analysis of transcriptional responses to methylglyoxal suggests that phoP mutants are unable to appropriately respond to methylglyoxal-induced stress. Together, our data suggest that the major virulence factor PhoP is essential for Mycobacterium tuberculosis resistance to methylglyoxal toxicity in vivo.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297704 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297704 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1265740 ncbi.nlm.nih.gov/bioproject/PRJNA1265740 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 6 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE297704 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |