Data · dataset · 2026
SDRG data of Liu Li group
Listed in ScienceDB
1.
Description
Nucleic Acid ElectrophoresisValidation of SDRG expression in the Drosophila head;Identification of the SDRG mutant.2. Quantitative PCR (qPCR)Detection of frq1 and frq2 RNA levels in the heads of wild-type and SDRG mutant flies;Detection of frq1 and frq2 RNA levels in the heads of flies following individual knockdown of candidate SDRG-binding protein-coding genes.3.
Neuromuscular Junction (NMJ) ImmunohistochemistryPhenotypic analysis and quantification of NMJ synaptic terminals in third-instar larvae of wild-type and SDRG mutants;Phenotypic analysis and quantification of NMJ synaptic terminals in third-instar larvae following individual knockdown of candidate SDRG-binding protein-coding genes;Phenotypic analysis and quantification of NMJ synaptic terminals in third-instar larvae following individual overexpression of frq1and frq2;Genetic interaction analysis between SDRG and frq1/ frq2;Genetic interaction analysis between the candidate protein Cora and frq1/ frq2;Genetic interaction analysis between the candidate protein Hrb and frq1/ frq2.4.
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RNA Immunoprecipitation (RIP)Screening for interaction sites between Cora and SDRG following SDRG overexpression in the Drosophila nervous system;Screening for Cora binding sites on frq2 RNA in wild-type flies;Comparative analysis of the binding occupancy at positive Cora–frq2 RNA interaction sites between wild-type and SDRG mutant flies.5. RNA Pull-Down coupled with Mass SpectrometryScreening of SDRG-interacting proteins from Drosophila heads using biotin-labeled sense probes and cold competitor probes.
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.41434 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Genetics 71%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.41434 | 8 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (71%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |