16S sequenceing date for cecal microbiota of pullets under acute heat stress

Many studies have reported that chlorogenic acid (CGA) has positive effects in attenuating heat stress. This study examined the effects of dietary supplementation with CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups.

The control group (C) and heat stress group (T1) received a basal diet. T2 group and T3 group received a basal diet supplemented with 300 mg/kg and 600 mg/kg CGA respectively, for two weeks before acute heat stress exposure. Pullets of T1, T2, and T3 group were exposed to 38℃ for 4 h while the control group was maintained at 25℃. 16 S rDNA Gene Sequencing for Cecal Microbiome was performed an Illumina Miseq.